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Modified Gomori trichrome staining Consecutive 12-m sections of unfixed fresh-frozen muscle (WT, Alix / , and ccOzz / ) were prepared on a cryostat, stained with Harris hematoxylin for 5 min, washed with water, and then stained with modified Gomori trichrome stain (0.6 g chromotrope 2R, 0.3 g Fast Green FCF, 0.6 g phosphotungstic acid, 1 mL glacial acetic acid in 100 mL deionized water)