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Description
Western blotting Protein samples were separated by electrophoresis and transferred to a nitrocellulose membrane (Millipore, USA), blocked with 5% non-fat milk for 2 h, and then incubated overnight at 4 C with primary antibodies specific for PCNA (WANLEIBIO), cyclin A (WANLEIBIO), CDK2 (Bioss), TGF- (Bioss), Caspase 3 (Bioss), Bax (Boster), Bcl-2 (Bioss), MMP2 (Bioss), MMP9 (Bioss), AMPK1 (Bioss), HSP47 (Boster), NLRP3 (WANLEIBIO), Caspase 1 (WANLEIBIO), IL-18 (Affinty), and IL-1 (Bioss)
Institutional animal care and use committee statement: Male Albino Wistar, body weight 200 g, 12 weeks old, randomly assigned, used in all of the experiments, 6 rats/group/interval, approved by local Ethic Committee

Farmacocintica No se localiz farmacocintica humana validada (vida media, biodisponibilidad) en fuentes revisadas por pares para ninguno de los tres componentes en esta investigacin

Pickova D, Ostry V, Toman J, Malir F

Giordano CR, Terlecky LJ, Bollig-Fischer A, Walton PA, Terlecky SR (2014) Amyloid-beta neuroprotection mediated by a targeted antioxidant

The half-life of T4 is very long, about 7 days, so a slow-release formulation is not needed
