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Subsequently, samples were sonicated to ensure complete disruption of all cells using a sonication bath (Transsonic 460, Elma Sonic, Singen, Germany) for 5 min at the highest frequency on ice

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A 10 L aliquot from each dilution was spotted onto 7H9 agar plates supplemented with or without 5 g/mL rifampicin

The involvement of mitochondria in ferroptosis as an important source of ROS is still the current mainstream view, since mitochondrial ROS (mitoROS) is mainly produced by oxidative phosphorylation (OXPHOS) complexes in mitochondria ( Although mitochondria are closely related to ROS production and iron metabolism, the causal relationship between morphological changes of mitochondria and ferroptosis remains unclear

[DOI] [Google Scholar] 251.Satoh-Nagasawa N., Mori M., Nakazawa N., Kawamoto T., Nagato Y., Sakurai K., Takahashi H., Watanabe A., Akagi H
