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Human hepatocellular liver carcinoma cell line HepG2 was bought from Shanghai Bioleaf Biotechnology Company (Shanghai, China) and human normal hepatocytes L-02 was from Silver Amethyst Biotech

Heme oxygenase - 1 Heme oxygenase (HO) plays a crucial role in regulating oxidative stress by maintaining heme homeostasis

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Since DFO is membrane impermeable and accumulates in lysosomes 18 , we believe that it protects the cells against ferroptosis by chelating lysosomal iron as described previously 47