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To overexpress the wild-type enzyme and its variants, 10 mL of an overnight culture (16-18 hours) was inoculated into 1 L of Luria-Bertani broth supplemented with 50 mg/mL kanamycin and 34 mg/mL chloramphenicol
doi: 10.1189/jlb.0912433
S., Allen, T
If you kill millions of fat cells, their stored triglycerides have to end up somewhere
Also, all affected HSP70s were more highly induced after 9 h than after 1 h in our study
doi: 10.1016/j.dnarep.2020.103032 118 OlcinaMMGrandRJHammondEM
