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doi: 10.3390/jcm10184184 176 WangYCuiCZhaoWTianXLiuPWeiLet al

Then, wells were washed three times with PBS-T and bound PDGF-BB and IL-1Ra variants were detected using biotinylated antibodies in PBS-T containing 0.1% BSA

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To understand the effect of host cell glutathione on existing infections, additional experiments were conducted in which A6 cells were supplemented with 2.5 mM cysteine at 24 hr post infection

As this oxidation does not occur in dead cells (no green fluorescence), this staining was also used to evaluate cell loss

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