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To be used for the experiments, the lyophilized powder was initially dissolved in NH 3 OH 6 M to obtain a stock solution with a final concentration of 250 M

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Increased knowledge of how bacteria and bacterial products such as endotoxin cross the epithelial barrier should yield data that facilitate the discovery of treatments aimed at abrogating the most feared complications of systemic endotoxemia, namely SIRS and MOF, whereas further study of paracellular permeability could likely lead to breakthroughs in the prevention of toxigenic diarrhea, a cause of substantial morbidity and mortality worldwide [87]