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Cells were washed and fixed with methanol (100%) and permeabilized with Triton X-100 (0.5%) in PBS

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Meanwhile, the Folate, having given up its methyl group, reverts to a base state so it can go pick up another methyl group and start the cycle all over again

Contribution of carrier-mediated transport systems to the blood-brain barrier as a supporting and protecting interface for the brain