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Lysosomal integrity survival screen sgNT K562 dCas9-KRAB and sg NPC1 K562 dCas9-KRAB cells were infected with genome-wide sgRNA library lentivirus to target 1,000 coverage of all guides at a low MOI to ensure roughly 1 guide per cell

Crooker K, Aliani R, Ananth M, Arnold L, Anant S, Thomas SM

Epithelial-to-mesenchymal transition induces cell cycle arrest and parenchymal damage in renal fibrosis

After treatment, the samples were fixed with 4% paraformaldehyde, rinsed with phosphate buffer solution, and then permeabilized with 0.1% Triton-X-100

These previous and current studies point towards the requirement of proactive research in the immunopathology caused by COVID-19
