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2.6 Real-time PCR analysis Total RNA was prepared from liver tissues and LO2 cells using the simple RNA Extract kit (Tiangen Biotech, China) and reverse transcription of RNA to cDNA using Fast King cDNA First Strand Synthesis Kit (Tiangen Biotech, China)

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PMCID: PMC8653225

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The effects of ALCAR on the induction of apoptosis and cell cycle arrest were investigated by flow cytometry (FC)

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