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doi: 10.1371/journal.pone.0104771 30 DiaoHKangZHanFJiangW

Glossary 2OG, 2-oxoglutarate

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The effect of ketamine on opioid-induced acute tolerance: can it explain reduction of opioid consumption with ketamine-opioid analgesic combinations

hPSCs were dissociated into single-cell suspensions using TrypLE Select (Thermo Fisher Scientific, Netherlands) and plated at a density of 2 10 6 cells/well in Stem Flex medium (Thermo Fisher Scientific, Netherlands) supplemented with 10 M Y-27632 (Peprotech, USA) on Geltrex-coated 6-well plates (Eppendorf, Germany) under a 5% CO 2 atmosphere