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Additional studies that incorporate parameters such as buffer type, surfactants, salt concentration, pH and other excipients should be evaluated for their impact on the proposed detection mechanism

Purity, sterility, dosing accuracy, and ingredient quality may vary significantly between sources

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These shifts in microbial composition can influence how the body responds to treatments, such as chemotherapy, antibiotics, and immunotherapy

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However, growing evidence indicates that maternal gut microbiota can modulate nutrient absorption, metabolic conversion, and subsequent deposition into eggs, giving rise to the concept of a gut-egg axis. While microbial variability introduces additional complexity and inter-individual variation in enrichment outcomes, it also presents new opportunities for targeted intervention