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Piperine enhances carbohydrate/fat metabolism in skeletal muscle during acute exercise in mice

If subjects experienced side effects of the noted treatment including, but not limited to, nausea, vomiting, diarrhea, or fever, they were instructed to stop treatment

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Cells were lysed with NIB-250 buffer (15 mM Tris-HCl (pH 7.5), 60 mM KCl, 15 mM NaCl, 5 mM MgCl 2 , 1 mM CaCl 2 , 250 mM sucrose, 1 mM DTT, 10 mM sodium butyrate, protease inhibitors) with 0.1% NP-40 for 5 minutes on ice

doi: 10.1016/j.exger.2013.02.012