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Cell pellets were then lysed using EmulsiFlex-C3 in a buffer containing 100 mM HEPES-KOH pH 7.5, 300 mM KCl, 2 mM dithiothreitol (DTT), 5 mM MgCl 2 , 5 mM imidazole, 10% glycerol, 0.1% IGEPAL CA-630 and protease inhibitor cocktail
cIAP1 and cIAP2 produce polyubiquitin chains that help recruit other components of complex I, like the linear ubiquitin chain assembly complex
