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Another translational gap arises from the limited generalizability of preclinical models [82]

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( I ) cAMP levels in cell lysates were determined following pretreatment with 100 nM each of GLP-1(2836), scrambled control, or forskolin in siRNA-treated cells (white bars) versus the scrambled siRNA control (gray bars) ( n = 3/group for all cAMP and LDH assays, each in triplicate)