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Description
In brief, cells were washed once with PBS and lysed by the addition of luciferase lysis buffer (1% Triton X-100, 25 mM Glycyl-Glycin (pH 7.8), 15 mM MgSO 4 , 4 mM EGTA, 1 mM DTT)
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Famularo G, Tzantzoglou S, Santini G, Trinchieri V, Moretti S, Koverech A, De Simone C
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