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MC4R mutations are the most frequent cause of monogenic obesity in humans (75)
Ryan M, McInerney D, Owens D, Collins P, Johnson A, Tomkin GH
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The Na + -free bath solution contained (mmol/l): 148.4 NMDG, 4.4 KHCO 3 , 1.2 KH 2 PO 4 , 2.6 CaCl 2 , 1.2 MgCl 2 , 10 HEPES (pH 7.4 with HCl)
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