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3,3,5,5- tetramethylbenzidine (TMB) substrate was added and the absorbance was read at 450 nm after 1 M HCl addition

This is particularly relevant for researchers studying mechanisms of insulin secretion improvement in type 2 diabetes models

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[DOI] [PMC free article] [PubMed] [Google Scholar] 7.Kopp KO, Glotfelty EJ, Li Y, Greig NH

2 Hypogonadotrophic hypogonadism Hypogonadotrophic hypogonadism is a key central endocrine cause of male infertility and is characterised by impaired hypothalamic gonadotropin-releasing hormone secretion
