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In general, protease was incubated with the test compound for 20 min before addition of fluorogenic substrate to initiate the reaction
doi: 10.1038/srep27096 144 ZhuZ.LiuL
doi: 10.1210/jc.2016-3842
doi: 10.1111/j.1528-1167.2011.03033.x 13 ArranzA
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The primary preclinical evidence comes from mouse studies showing reduced body weight and adipose mass in diet-induced obese mice (2018), improved grip strength in aged mice (2024), and beneficial microbiome changes when combined with caloric restriction (2022)
