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The bacterial cells were harvested, resuspended in binding buffer (20 mM sodium phosphate, pH 7.4, containing 500 mM NaCl and 20 mM imidazole), and lysed by treatment as above, followed by sonication
Passwater, Taking the Confusion Out of Selenium, WholeFoods Magazine 38 (1), 4445, 48 (2015), www.wholefoodsmagazine.com/columns/vitamin-connection/taking-confusion-out-selenium
