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This is only a measurement of the effects of secondhand smoke

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Quantitative real-time PCR of DAB2IP transcript was carried out on StepOnePlus Real-time PCR System (Applied Biosystems, Foster City, CA, USA) using specific FAM-labelled probe and primers (revalidated TaqMan Gene expression assay for DAB2IP Hs00368995, Assays on Demand, Applied Biosystems)

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View at Publisher | View at Google Scholar Hashibe M, Brennan P, Benhamou S, Castellsague X, Chen C et al

"That's more than 47,000 who are not here with us to enjoy this morning," Yerger said during a briefing