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The serum and urinary concentrations of cotinine, a stable metabolite of nicotine, were determined using a mouse/rat cotinine ELISA kit (Calbiotech, San Diego, CA) following the manufacturer's instructions

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Elway was sacked 516 times, second to Favre for most times sacked in NFL history

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Longitudinal intervention cohorts can provide more granularity as to how intervention effects may be attenuated or strengthened over time as well as insights into critical life course events at distinct ages