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(91) found that the destruction of vagal afferent fibers by capsaicin or lesions to subdiaphragmatic vagal fibers abolished ghrelin-induced feeding and substantially decreased c-fos expression in the ARC, where vagal afferents terminate, in response to ghrelin in capsaicin-treated rats

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Subsequently, the cells were washed three times with assay buffer to remove the ligand and allowed to equilibrate in assay buffer for 30 min before being stimulated a second time with a fixed concentration of Ex4 at an EC 90 (5 in assay buffer)

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