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Briefly, total RNA (1 g) was denatured at 65 C for 5 min together with random hexadeoxynucleotide primers (2.5 M)
A pesar de los miles de reportes anecdticos positivos y los estudios exitosos en modelos animales, la evidencia en humanos todava es limitada
doi: 10.1002/glia.22558 139
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10.12688/f1000research.9970.1 46 LeeM
(2) Add 300 L of protein precipitant methanol-acetonitrile (V:V=2:1, containing mixed internal standard, 4 g/mL), vortex and shake for 1 min
