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Cells were then transferred to ice, washed twice with cold Dulbeccos phosphate-buffered saline, and lysed in 180 l SDS Loading Dye (Cell Signaling Technologies, Danvers, MA)

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Ann Neurol 2013;74(2):199208

One additional challenge is identifying specific and reliable markers that are exclusively expressed on senescent cells to ensure accurate targeting by CAR-T cells or antibodies or to use for immunization
