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clinical relevance

The aortas were then incubated in freshly prepared SA--Gal staining solution at pH 6.0 overnight at 37 C, according to the manufacturers protocol

Testing should also consider possible iron deficiency anaemia, macrocytosis, and take into account people on a vegan diet and individuals with a gastrointestinal disorder associated with a vitamin B12 deficiency (see Table 2)[17]

Although ZO1 displacement is not sufficient to cause a barrier defect, the combination with other signalling pathways affecting tight junction structure, may contribute to a rearrangement of junctional complex, impacting on tight junction stability (114)

To detect changes between groups within timepoints, two-way ANOVAs were performed at each timepoint using Tukeys post-hoc test