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Cell culture and cigarette smoke extract (CSE) stimulation 16HBE cells were cultured in RPMI 1640 medium (Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillinstreptomycin (Gibco)

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In Europe, the MOE was introduced in 2005 as the preferred method for risk assessment of carcinogenic and genotoxic compounds 19

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The law covers e-cigarettes, cigarettes, cigars, pipe tobacco, vape pens, vape oils and all tobacco-related products