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Secreted insulin was analysed from a sample of diluted supernatant by immunoassay (Cisbio wide range HTRF assay, measured using a Spectramax i3x plate reader)

This integration exemplifies process analytical technology (PAT) and quality by design (QbD) principles, preventing batch deviations and ensuring consistent product quality from the outset [499, 500]

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[DOI] [PMC free article] [PubMed] [Google Scholar] 56.Christensen S, Robinson K, Thomas S, Williams DR

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