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After completion, as monitored by UPLC and ESI-LCMS, the reaction mixture was diluted with buffer A, filtered and purified directly by preparative RP-HPLC, eluting with a linear gradient (see the Peptide cleavage and purification section)

Given the impact of androgen excess on multiple reproductive and metabolic parameters of females, such analyses are deemed mandatory in order to define the potential utility of such compounds for the personalized treatment of women suffering from PCOS

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MNT spoke with Hsinlin Thomas Cheng, MD, PhD, senior neurologist in the Department of Neurology at Massachusetts General Hospital and assistant professor at Harvard Medical School, about this study

Most researchers choose between two common approaches