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Apart from the -helical domain of G s and the extracellular domain (ECD) of GIPR, the presence of retatrutide, each individual receptor and heterotrimeric G s in the respective complexes were clearly visible in all three cryo-EM maps, thereby allowing unambiguous modeling of the secondary structure and side-chain orientation of all major components (Supplementary Fig

From these data, we conclude that GLP-1R is involved in regulating TNC microglial activation during NTG injection, and this function of GLP-1R might also be the mechanism for attenuating CM-related allodynia

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