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By utilizing the home-made reversed-phase column, we then conducted Liquid Chromatograph Mass Spectrometry (LCMS/MS) analysis was later conducted with a gradient of solvents A (2% acetonitrile, 0.1% formic acid within water) and B (90% acetonitrile, 0.1% formic acid within water) using the EASY-nLC 1200 UPLC system

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71 A review of nonpharmacological interventions for people with dementia found that the strongest evidence for reducing responsive behaviors (behaviors in response to sensations such as pain, hunger, thirst, fatigue, and feeling too hot or too cold) involved music, sensory stimulation, simulated presence and validation therapies