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The concentration of each of these labeled metabolites was measured against norvaline as an internal standard
Several variables influence your timeline

Activation of mTORC1 leads to the phosphorylation of S6 kinase-1 (S6K1) and eukaryotic translation initiation factor 4E-binding protein-1 (4EBP1), which stimulates protein translation and cell growth (Wolfson and Sabatini, 2017)
