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It is primarily caused by autoimmune destruction of the gastric parietal cells, leading to a deficiency in Intrinsic Factor and, consequently, a failure to absorb vitamin B12
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After a wash step, a horseradish peroxidase (HRP) conjugated murine anti-AMG 479, clone B35 at 400 ng/mL was added to the plate and incubated for 1.5 h

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