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Forty-eight hours after transfection, the cells were washed twice in binding buffer (50 mM HEPES, pH 7.4, supplemented with 1 mM CaCl 2 , 5 mM MgCl 2 and 0.5% (w/v) BSA) and incubated for 15 min at 4 C

Thus, variations of each single factors could potentially explain the discrepancies observed in the different studies (66)

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The good news is that adaptation at higher doses typically happens faster than the initial adjustment

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