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To stain lipids, 1 g/mL Nile Red (AAT Bioquest, USA) was added to the medium, and cells were incubated at 37 C for 15 min, then washed with PBS and fixed
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Obesity is another factor that can increase sympathetic activity and oxidative stress
The addition of vitamin B 12 mimics has also been observed by other research teams to reduce feedback inhibition and boost cobalamin synthesis
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