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RT-qPCR The total RNA of cultured epithelial cell lines was extracted by using TRIzol (Invitrogen, CA, USA) reagent according to the manufacturers protocol

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[69] 2008 Obama cover satire and controversy [edit] On July 21, 2008, "The Politics of Fear", a cartoon by Barry Blitt, was featured on the cover of The New Yorker , depicting then presumptive Democratic presidential nominee Barack Obama in the turban and shalwar kameez typical of many Muslims, fist bumping with his wife, Michelle, portrayed with an Afro and wearing camouflage trousers with an assault rifle slung over her back