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As the exendin-4 ELISA above was not sensitive enough to detect circulating drug levels at this dose, a separate cohort of lean animals received 2.4 nmol 1 kg 1 day 1 agonist via osmotic minimpump and, with samples taken after 16 days and exendin-4 levels analyzed as above
( 10.1016/S2213-8587(2100203-5) [DOI] [PubMed] [Google Scholar] Schilling JM Roth DM & Patel HH
The starting dose is not just a formality
