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Each liver was homogenized in 3ml of 0.25 M sucrose per g of liver, and centrifuged at 3,000 and 10,000 rpm for 10 minutes respectively to precipitate cell debris

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2.2 Determination of physiological and biochemical parameters On the 12th day of drought stress, three Astragalus plants per treatment with similar growth vigor were selected to measure plant height (PH) and stem diameter (SD), with fresh apical leaves harvested and immediately stored at -80 C for physiological and transcriptomic characterization

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