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Once breakdown starts, a protein called intrinsic factor, released by parietal cells in the stomach, binds to vitamin B12 in the duodenum (small intestine) and forms a B12-intrinsic factor complex that allows B12 to be absorbed in the terminal portion of the small intestine

Adewale OB, Anadozie SO, Okpiri RT, Jaiyesimi KF, Owolabi OV, Akinlade O et al (2022) Synthesized gold nanoparticles mediated by Crassocephalum rubens extract down-regulate KIM-1/NGAL genes and inhibit oxidative stress in cadmium-induced kidney damage in rats

Some research from Germany has shown that an even higher threshold (over 500 pg/mL) is necessary in early-to-mid pregnancy to maintain vitamin B12 levels throughout pregnancy, lactation, and to support an infants vitamin B12 status (via breast milk) through 6 months of age
