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Description
Briefly, Nb35 target gene was transformed in BL21 and grown in TB culture medium with 100 g/mL ampicillin, 2 mM MgCl2 and 0.1 % (w/v) glucose at 37C, 180 rpm

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doi: 10.2337/dci18-0018

Contact your GP before stopping if you experience persistent side effects, are planning pregnancy, or have concerns about long-term use or treatment goals
