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cDNA was then used as template for RT-qPCR with a QuantStudio 3 Real-Time PCR system (Applied Biosystems) using PowerUP SYBR Green Master Mix (Applied Biosystems) and 500 nM of target gene specific forward and reverse primers, using the recommended fast cycling mode

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This will encourage cellular recovery as much as possible

An RGD spacing of 440 nm is sufficient for integrin alpha V beta 3-mediated fibroblast spreading and 140 nm for focal contact and stress fiber formation