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Primary Human Hepatocytes (PHHs) are considered the gold standard of vitro models for predicting in vivo metabolism because they can mimic hepatic metabolic reactions, including those catalyzed by cytochrome P450 (CYP) enzymes and by flavin-containing monooxygenases (FMOs), as well as conjugation with glucuronic acid, sulfate, and glutathione to a large extent

The working hypothesis, discussed in the Giblin et al

Taken together, these findings illustrate a role for GPx-1 in modulating mitochondrial generation of oxidants that regulate redox-dependent processes involved in growth factor signal transduction

However, this is very rare since glutathione is a naturally occurring substance in the body and usually happens due to high doses of glutathione

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