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Then substrate A (50 l) and substrate B (50 l) were added to each well, gently mixed, and incubated for 15 min at 37 C in dark

Simultaneously, NAD+ levels increased by 1.2 to 1.6 fold compared to untreated cells

doi: 10.1038/ejcn.2017.131

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Additionally, changes also occurred in the distribution of the sleep stages REM and N3 within the sleep cycle