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What the margin difference looks like in practice: Community sources describe the room-temperature-stored punctured vial as more likely to reach the boundary of preservative efficacy within the 28-day window still technically within the documented period, but with less safety margin

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First, insomnia was determined subjectively using self-reported sleep measures, which may be affected by estimation bias compared to more-objective measures of sleep, such as actigraphy and polysomnography