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The column was pre-equilibrated with 10 column volumes (CV) of 1 Dulbeccos Phosphate Buffered Saline (DPBS) (pH 7.4) (Sigma-Aldrich)/0.05% (v/v) sodium azide (Sigma-Aldrich), before the supernatant was loaded onto the column at a flow rate of (0.52) mL/min

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Within 2 hours of waking: a small protein-forward eat (even if its modest)