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But, if your yields are substantially lower than that, and youre not working with a big GST-fusion protein, then it is worth considering how you can optimize the binding buffer during the capture step

when the amount of the reduced glutathione for injection is 1.0g, the formula components are as follows: 1.0g of reduced glutathione, adjusting the pH value to 6.0-7.0 by using 10% sodium hydroxide solution, and adding 3.3g of water for injection