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To expand on the chemistry of attaching ligands to agarose beads a little, there are usually 3 components to the ligand (Figure 3): the ligand itself that binds to the peptide or protein tag the reactive chemical group that forms a covalent bond with the ligand a spacer that connects the reactive chemical group to the agarose bead Figure 3

You dissolve the desired amount of agarose in liquid, heat it up, then let it cool down into the shape that you desire

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