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For genotyping, DNA from toe clipping of littermate animals was extracted using a house-made Lysis buffer (0.1 M Tris-HCl pH 7.5, 0.1 M EDTA, 0.1 M NaCl, 1% SDS), and the PCR was performed using primers described in Supplementary Dataset 5 and the products were analyzed on an agarose gel

The primary difference is chemical stability

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This threshold was then kept the same for all images of each experiment

Experimental evidence for ascorbate-dependent electron transport in leaves with inactive oxygen evolving complexes

pylori eradication to assess whether H