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Exp Cell Res (2002) 276(1):2431

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The HPLC system was equipped with a C18 reversed-phase column and a detector suitable for amino acid detection (e.g., fluorescence or ultraviolet detector), and a gradient elution protocol was applied using a buffer (e.g., sodium acetate solution) and methanol

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Every visit includes screening (history, meds, allergies), sterile IV placement by a licensed RN, and monitoring throughout